Real Time PCR NRTP-501 offers reaction volumes ranging from 1 to 50μl, making it suitable for various applications. It utilizes a Peltier-based heating and cooling system for precise temperature control. This unit features a 96-well capacity for high-throughput sample processing. It includes six durable, high-efficiency LEDs for consistent fluorescence excitation. Our PCR system is designed with user-friendly navigation to ensure smooth operation.
$21,000.00
Specifications
| Sample capacity | 96 wells |
| Reaction volume | 1 to 50μl |
| Heating/cooling method | Peltier |
| Maximum ramp rate | 6℃/s |
| Temperature setting range | 4 to 100℃ |
| Temperature accuracy | ±0.2℃ |
| Temperature uniformity | ±0.2℃ |
| Gradient zone | 12 columns |
| Gradient range | 1 to 36℃ |
| Heated lid | Electronic automatic lid |
| Excitation source | 6 long-life, high-performance LEDs |
| Detector | Highly sensitive PMT (photo multiplier tube) with a Fresnel lens |
| Scanning principle | Time-resolved scanning technology |
| Detector position | Top of the block |
| Excitation/detection range | 455 to 650nm/510 to 715nm |
| Fluorescence channel | 6 |
| Detection sensitivity | 1 copy of the target sequence |
| System sensitivity | Detect differences as small as 1.33-fold in target quantities in singleplex reactions |
| Dynamic range | 10 orders of magnitude |
| Dye compatibility | FAM/SYBR Green, VIC/JOE/HEX/TET, NED/TAMRA/Cy3 JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Data analysis modes | Absolute quantification, Relative quantification, Endpoint qualitative analysis, Melt curve analysis, Protein Stability Screening, Genotyping |
Features
Applications
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FAQ for Real Time PCR NRTP-501
1: How does the Real Time PCR NRTP-501 ensure high-quality data?
Our Real Time PCR system combines innovative optics with stable thermal control to deliver precise quantification and amplification. The system supports a 10-log dynamic range and can detect 1.33-fold differences in target levels.
2: Can this Real Time PCR system be used for multiplex assays?
The Real Time PCR system allows true five-target multiplexing in a single well with minimal signal interference. This makes it ideal for complex experiments requiring simultaneous detection of multiple targets.
3: What is the benefit of the Fresnel lens in this Real Time PCR NRTP-501?
The Real Time PCR system integrates a Fresnel lens with a short focal length to collect more light while minimizing signal loss. This design improves sensitivity and reduces optical noise.
4: Can the Real Time PCR NRTP-501 perform melt curve analysis for specificity checks?
Our Real Time PCR system includes a robust melt curve module for post-amplification analysis. This allows you to verify product specificity and identify non-specific products or primer-dimers.
5: What routine care should be performed by users?
It is important to keep the block clean and ensure that no liquid spills or debris accumulate in the well area. Wipe the surface gently with a lint-free cloth and follow the cleaning guidelines in the user manual.
6: How accurate and reproducible are the results from this Real Time PCR system?
This system delivers high reproducibility with minimal variation between replicates. Its uniform thermal block design ensures consistent amplification across all wells, which is crucial for sensitive Real Time PCR analysis, including low-copy target detection.
7: How user-friendly is the software interface of this Real Time PCR system?
The software is designed for ease of use, featuring a navigational interface and project management tools. Users can quickly load saved protocols, import sample information, and initiate Real Time PCR runs with minimal input, reducing setup time and human error.
8: Is this Real Time PCR system suitable for point-of-care or field use?
Absolutely. Its lightweight, portable design and battery-powered operation make this Real Time PCR system perfect for point-of-care testing and mobile diagnostic units. It is engineered for stable performance even in remote or non-laboratory settings.
9: What steps should I take if the Real Time PCR system shows inconsistent results?
Ensure that sample volumes are uniform, reagents are fresh, and the instrument is placed on a stable surface. Verifying thermal cycling parameters and proper sealing of reaction tubes can also help improve Real Time PCR consistency.
Labnics Real Time PCR use fluorescent systems for accurate real-time DNA quantification. They support multiplex detection for simultaneous amplification of multiple targets. These units offer multi-melting curve analysis for reliable result confirmation. They come with built-in software for streamlined data analysis and reporting. Our Real-Time PCR includes data export capability for efficient result management and analysis.